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- Total RNA was extracted from embryos working with ISOGEN and ready for cDNA synthesis utilizing SuperScriptHII transcriptase
- Total RNA was extracted from neuroblasts using TRIzol reagent according to the manufacturer's instructions
- Total RNA was extracted using a modified version of the TRIzol method (Invitrogen), in which RNA is purified directly from the aqueous phase using the Rneasy MinElute RNA purification kit
- Total RNA was isolated from each C. elegans sample using a combination of TRIZOLH (Invitrogen, Karlsruhe, Germany) till the ethanol precipitation step followed by purification via the RNeasy Mini Kit
- Total RNA was isolated using the TRIzol reagent (Invitrogen, Carlsbad CA, USA) according to the manufacturer's instructions
- Total RNA was reverse transcribed with oligo primer employing the M-MLV reverse transcriptase for RTPCR
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- Total cell lysates were resolved by SDS-PAGE and immunoblotted with antibodies against PDCD4 or tubulin
- Total energy intake is an important confounder in epidemiological research assessing the association between diet or nutrition and chronic diseases
- Total protein was quantified by the Bio-Rad protein assay, and processed for Western blot using primary goat antibodies for VCAM-1 and ICAM-1
- Total scores of 3 were defined as positive to simplify data analysis. To analyze the correlation between CEACAM6 and E-cadherin expression
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